Background: It has been proven that cytokines have a role in cancer immunity and carcinogenesis in general, which have a major role in causing many types of solid cancer. This study aimed to estimate the levels of IL-5 and IL-4 in the blood of some diagnosed breast cancer patients, and to study the relationship of these interleukins to the development of human breast cancer. Patients and methods: The study included 75 women, divided into two groups, including 50 breast cancer patients and 25 other apparently healthy women. The ELISA method was used to estimate the level of IL-5 and IL- 4 in the blood serum of the two groups studied. Results: The current study showed an increase in the level of interleukin 4 in the blood of breast cancer patients, with significant differences between breast cancer and the control group (p<0.005), and this increase was associated with tumor development. In addition, it was found that high levels of IL-4 in the blood were directly associated with increasing of Ca15.3. On the other hand, a significant decrease in the average level of interleukin 5 in blood serum was observed in affected patients compared to control groups. Conclusions: The study showed an association between high levels of IL- 4 in the blood and breast cancer, and it was found that this increase is related to the advanced stage of the disease. In addition, from our study there is no statistically significant association between a statistically significant decrease in the level of serum IL-5 and the advanced stage of human breast cancer.