Most researchers usually investigate the concentration of chemical components in the blood of people suffering from a disease, and studies are rarely conducted in healthy people. So the researchers conducted this preliminary statistical study of both creatinine and some electrolytes in the blood serum of a group of healthy males. Years 25 samples of healthy male blood were collected in Sabratha city. Their average age was 13.64, 41.92, The concentration of sodium, chloride, potassium., magnesium ions, and creatinine ions were measured in the blood serum of each volunteer in the hospital the city of Sabratha. The mean and deviation were standard for the concentrations of these chemical components, respectively: (5.15, 140.62 mmol /L, 3.98 (106.80) ) mmol /L, 0.74 4.60 ) mmol /L, 0.18 2.06) milligram / dL ), 0.17 0.84) milligrams / dL. When compared to internationally accepted reference values, the results showed the necessity of working to determine the concentration of creatinine, sodium, and potassium. And chloride through the serum of healthy males in Sabratha and then Libya. In the case of magnesium, it seemed clear that the internationally accepted reference value was sufficient for the purpose. The limits of the reference values were determined for the concentration of sodium, chloride , potassium ions , and the concentration of creatinine , and they were, respectively, (126.6, 149.8) mmol /L, (97.1,114.7) mmol /L, (2.9,6.0) mmol /L, (0.4,1.1) Milligrams / dL, and these limits were wider compared to the limits of reference values used in laboratories. The type of relationship between the study variables was also determined using the person correlation coefficient) , and this relationship) was confirmed graphically using what is known as in kilograms , the concentration of sodium and chloride ions was more interconnected and similar.