The aim was to assess women's knowledge, attitudes and practices regarding cervical cancer screening. Materials and methods: We conducted a descriptive and analytical study at the Reference Health Center of Commune V of the District of Bamako. Result: The average age of women was 30 years. They were mostly married (81.79%), primigestes (50.17%), nulliparous (58.76%), uneducated (53.61%) and living in urban areas (62.80%). Most of the women had already heard of cervical cancer (79.90%). Only (8.59%) of women recognized STIs as a risk factor for cervical cancer and 44.70% knew that cervical cancer was preventable. Of the women surveyed, none had already been vaccinated and about 60% had never been tested. 15.46% of women wanted to get the vaccine soon, compared to only 8.59% now. 41.20% of women had already been screened, 36.40% of them wanted to do it now against 70.80% soon.78% of women said they wanted to recommend screening to a relative. Conclusion: Most women have knowledge of its existence but their knowledge of risk factors and means of prevention is low. Their attitudes and practices are also weak.